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marker isolectin b4  (Vector Laboratories)


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    Structured Review

    Vector Laboratories marker isolectin b4
    Marker Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 667 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/griffonia+simplicifolia+lectin+i/Biotinylated+Griffonia+Simplicifolia+Lectin+I+(GSL+I)+isolectin+B4/pmc13133793-44-48-54
    Average 95 stars, based on 667 article reviews
    marker isolectin b4 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Sexually dimorphic role of estrogen receptor α in preserving right ventricular endothelial integrity
    Article Snippet: Apoptotic cells were detected using In Situ Cell Death Detection Kit (Roche) following the manufacturer’s protocol. .. After completion of TUNEL labeling, slides were incubated overnight at 4°C with Griffonia simplicifolia lectin I (GSL I; Vector Laboratories) to visualize capillary structures. .. Slides were rinsed, mounted with ProLongTM Gold Antifade Mountant (Invitrogen), and imaged using an IX83 inverted fluorescence microscope (Olympus).

    Labeling:

    Article Title: Sexually dimorphic role of estrogen receptor α in preserving right ventricular endothelial integrity
    Article Snippet: Apoptotic cells were detected using In Situ Cell Death Detection Kit (Roche) following the manufacturer’s protocol. .. After completion of TUNEL labeling, slides were incubated overnight at 4°C with Griffonia simplicifolia lectin I (GSL I; Vector Laboratories) to visualize capillary structures. .. Slides were rinsed, mounted with ProLongTM Gold Antifade Mountant (Invitrogen), and imaged using an IX83 inverted fluorescence microscope (Olympus).

    Incubation:

    Article Title: Sexually dimorphic role of estrogen receptor α in preserving right ventricular endothelial integrity
    Article Snippet: Apoptotic cells were detected using In Situ Cell Death Detection Kit (Roche) following the manufacturer’s protocol. .. After completion of TUNEL labeling, slides were incubated overnight at 4°C with Griffonia simplicifolia lectin I (GSL I; Vector Laboratories) to visualize capillary structures. .. Slides were rinsed, mounted with ProLongTM Gold Antifade Mountant (Invitrogen), and imaged using an IX83 inverted fluorescence microscope (Olympus).

    Article Title: Fibroblast Has2 limits acute heart failure following myocardial infarction in male mice
    Article Snippet: The sections were then incubated in Wheat Germ Agglutinin‐Rhodamine (WGA; Vector Laboratories, Cat. #RL‐1022; 1:50 dilution) for 30 min at 37°C. .. After three 3‐min washes with 1X DPBS, the sections incubated in Griffonia simplicifolia Lectin I (GSL I) Isolectin‐B4‐Fluorescein (Vector Laboratories, Cat. #FL‐1201–0.5) diluted 1:25 in isolectin staining solution (1 mM CaCl 2 and 10 mM HEPES in 1× PBS) for 1 h at room temperature followed by three 3 min washes in 1× DPBS. ..

    other:

    Article Title: CHD7 regulates cardiac neural crest cell differentiation through SOX5-mediated self-activation
    Article Snippet: Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4, DyLight® 594 (DL-1207-0.5) , Vector Laboratories , CatDL-1207-0.5.

    Staining:

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure
    Article Snippet: After three washes in 0.1% Triton X-100 in PBS (5, 10, and 15 min), sections were incubated for 75 min at room temperature with Alexa Fluor-conjugated secondary antibodies (anti-rabbit A21245; anti-mouse A11031; both 1:300, Invitrogen) and lectins. .. Lectin staining was performed using Griffonia simplicifolia Lectin I (FITC, FL-1101, Vector Laboratories) for mouse samples and Ulex europaeus agglutinin I (UEA I, Alexa FluorTM 594 conjugate, L32476 , Invitrogen), respectively, for human samples. .. Nuclei were counterstained with DAPI (1:1000, D1306, Invitrogen) for 5 min, and coverslips were mounted with Dako Fluorescence Mounting Medium (S3023, Agilent).

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure.
    Article Snippet: After three washes in 0.1% Triton X-100 in PBS (5, 10, and 15 min), sections were incubated for 75 min at room temperature with Alexa Fluor-conjugated secondary antibodies (anti-rabbit A21245; anti-mouse A11031; both 1:300, Invitrogen) and lectins. .. Lectin staining was performed using Griffonia simplicifolia Lectin I (FITC, FL-1101, Vector Laboratories) for mouse samples and Ulex europaeus agglutinin I (UEA I, Alexa FluorTM 594 conjugate, L32476, Invitrogen), respectively, for human samples. .. Nuclei were counterstained with DAPI (1:1000, D1306, Invitrogen) for 5 min, and coverslips were mounted with Dako Fluorescence Mounting Medium (S3023, Agilent).

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure
    Article Snippet: .. Sections were stained with Wheat Germ Agglutinin (WGA, Texas RedTM, W21405 , Invitrogen), Griffonia simplicifolia Lectin I (GSL I, FITC, FL-1101, Vector Laboratories), and DAPI (D1306, Invitrogen). .. Imaging was performed on a PhenoImager Fusion microscope (Akoya Biosciences), and spectral unmixing was carried out using InForm software (Akoya Biosciences).

    Article Title: Fibroblast Has2 limits acute heart failure following myocardial infarction in male mice
    Article Snippet: The sections were then incubated in Wheat Germ Agglutinin‐Rhodamine (WGA; Vector Laboratories, Cat. #RL‐1022; 1:50 dilution) for 30 min at 37°C. .. After three 3‐min washes with 1X DPBS, the sections incubated in Griffonia simplicifolia Lectin I (GSL I) Isolectin‐B4‐Fluorescein (Vector Laboratories, Cat. #FL‐1201–0.5) diluted 1:25 in isolectin staining solution (1 mM CaCl 2 and 10 mM HEPES in 1× PBS) for 1 h at room temperature followed by three 3 min washes in 1× DPBS. ..

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure.
    Article Snippet: .. Sections were stained with Wheat Germ Agglutinin (WGA, Texas RedTM, W21405, Invitrogen), Griffonia simplicifolia Lectin I (GSL I, FITC, FL-1101, Vector Laboratories), and DAPI (D1306, Invitrogen). .. Imaging was performed on a PhenoImager Fusion microscope (Akoya Biosciences), and spectral unmixing was carried out using InForm software (Akoya Biosciences).

    Whole Genome Amplification:

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure
    Article Snippet: .. Sections were stained with Wheat Germ Agglutinin (WGA, Texas RedTM, W21405 , Invitrogen), Griffonia simplicifolia Lectin I (GSL I, FITC, FL-1101, Vector Laboratories), and DAPI (D1306, Invitrogen). .. Imaging was performed on a PhenoImager Fusion microscope (Akoya Biosciences), and spectral unmixing was carried out using InForm software (Akoya Biosciences).

    Article Title: Downregulation of TCF19 and ATAD2 causes endothelial cell cycle arrest at the transition from cardiac hypertrophy to heart failure.
    Article Snippet: .. Sections were stained with Wheat Germ Agglutinin (WGA, Texas RedTM, W21405, Invitrogen), Griffonia simplicifolia Lectin I (GSL I, FITC, FL-1101, Vector Laboratories), and DAPI (D1306, Invitrogen). .. Imaging was performed on a PhenoImager Fusion microscope (Akoya Biosciences), and spectral unmixing was carried out using InForm software (Akoya Biosciences).



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    Vector Laboratories ib4
    Refi - 10 reduces pathological neovascularization in oxygen-induced retinopathy (OIR) mice. ( A ) Refi-10 was administered intraperitoneally at P13, and retinas were analyzed at P17. ( B ) Analysis of retinal vasculature. ( a ) Representative <t>IB4-stained</t> whole-mount retinas from normal, OIR, OIR + Refi-10 (25 mg/kg), and OIR + Refi-10 (50 mg/kg) mice at P17. Avascular areas were circled in white . ( b ) Enlarged views of the boxed regions in a . Yellow arrows indicate vascular tufts. ( c ) Neovascularization regions were highlighted in white by ImageJ software. ( d , e ) The quantification of neovascular area and avascular area for each group. Scale bars = 1000 µm. ( C ) Representative H&E staining of retinal sections and quantification of endothelial cell nuclei anterior to the inner limiting membrane. Scale bars = 500 µm. Data are presented as mean ± SD ( n = 6 mice per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    Refi - 10 reduces pathological neovascularization in oxygen-induced retinopathy (OIR) mice. ( A ) Refi-10 was administered intraperitoneally at P13, and retinas were analyzed at P17. ( B ) Analysis of retinal vasculature. ( a ) Representative <t>IB4-stained</t> whole-mount retinas from normal, OIR, OIR + Refi-10 (25 mg/kg), and OIR + Refi-10 (50 mg/kg) mice at P17. Avascular areas were circled in white . ( b ) Enlarged views of the boxed regions in a . Yellow arrows indicate vascular tufts. ( c ) Neovascularization regions were highlighted in white by ImageJ software. ( d , e ) The quantification of neovascular area and avascular area for each group. Scale bars = 1000 µm. ( C ) Representative H&E staining of retinal sections and quantification of endothelial cell nuclei anterior to the inner limiting membrane. Scale bars = 500 µm. Data are presented as mean ± SD ( n = 6 mice per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
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    Image Search Results


    Refi - 10 reduces pathological neovascularization in oxygen-induced retinopathy (OIR) mice. ( A ) Refi-10 was administered intraperitoneally at P13, and retinas were analyzed at P17. ( B ) Analysis of retinal vasculature. ( a ) Representative IB4-stained whole-mount retinas from normal, OIR, OIR + Refi-10 (25 mg/kg), and OIR + Refi-10 (50 mg/kg) mice at P17. Avascular areas were circled in white . ( b ) Enlarged views of the boxed regions in a . Yellow arrows indicate vascular tufts. ( c ) Neovascularization regions were highlighted in white by ImageJ software. ( d , e ) The quantification of neovascular area and avascular area for each group. Scale bars = 1000 µm. ( C ) Representative H&E staining of retinal sections and quantification of endothelial cell nuclei anterior to the inner limiting membrane. Scale bars = 500 µm. Data are presented as mean ± SD ( n = 6 mice per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: An APE1 Redox Inhibitor Attenuates Pathological Retinal Vascularization by Suppressing FGF2 Angiogenic Signaling

    doi: 10.1167/iovs.67.3.47

    Figure Lengend Snippet: Refi - 10 reduces pathological neovascularization in oxygen-induced retinopathy (OIR) mice. ( A ) Refi-10 was administered intraperitoneally at P13, and retinas were analyzed at P17. ( B ) Analysis of retinal vasculature. ( a ) Representative IB4-stained whole-mount retinas from normal, OIR, OIR + Refi-10 (25 mg/kg), and OIR + Refi-10 (50 mg/kg) mice at P17. Avascular areas were circled in white . ( b ) Enlarged views of the boxed regions in a . Yellow arrows indicate vascular tufts. ( c ) Neovascularization regions were highlighted in white by ImageJ software. ( d , e ) The quantification of neovascular area and avascular area for each group. Scale bars = 1000 µm. ( C ) Representative H&E staining of retinal sections and quantification of endothelial cell nuclei anterior to the inner limiting membrane. Scale bars = 500 µm. Data are presented as mean ± SD ( n = 6 mice per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

    Article Snippet: Then, they were incubated with IB4 (1:100, DL-1208-.5; Vector Laboratories, USA) overnight at 4°C.

    Techniques: Staining, Software, Membrane